Method for the preparation of transgenic plants characterised by geminivirus lasting resistance
The invention relates to a process for obtaining transgenic plants resistant to geminiviruses in a lasting manner. The transgene for expression in plant consists of a polynucleotide sequence derived from pathogenic virus. The sequence of the pathogen is derived for example from the sequence encoding the "protein associated with replication" (Rep) of the virus. The protein expressed by this sequence acts in trans as a dominant negative mutant, interfering with viral replication. In order to avoid the post-transcriptional gene silencing operated by the virus on sequence homologous transgenic, the nucleotide sequence derived from the pathogen is suitably mutagenized, in order to reduce the homology with the corresponding viral sequence, but leaving unchanged the protein sequence encoded. The plants thus transformed are not subject to gene silencing and resist in a stable manner to viral infection. Inventors: Tavazza M., Noris E. Lucioli A., Accotto G.P., Tavazza R., Brunetti A. Years: 2003-2004 N°: RM2003A000242 – PCT/IT0400287 Patent: Italian – International
Single chain antibody (scFv) against Tospovirus and their use in diagnosis and therapy
Inventors: Franconi Rosella (ENEA), Benvenuto Eugenio (ENEA), Roggero Piero (IVV-CNR) Year: 1998 N°: SA/A/14810 Patent: Italian
Ulmus ‘ARNO’, an elm cultivar resistant to Dutch elm disease (DED)
‘Arno’ was selected from seedlings obtained by controlled pollination of the Dutch selction Ulmus ‘Plantijn’ with an individual of Ulmus pumila. Resistance to DED was verified by checking symptoms after artificial inoculations in two different years on 10 3-years-old rooted cuttings. ‘Arno’ is single stemmed (monocormic), with erect habit, ascending branches, and an upright oval crown. Field trials indicate that ‘Arno,’ although slower than ‘Fiorente,’ is among the fastest growers ever tested. The trunk is straight, branching at a height of 3 m. The wood characteristics of this clone are not different from those known for field elm (Santini et al. 2004). ‘Arno’ is named for the river that passes through the city of Florence. The main advantage in using ‘Arno’ is its resistance to DED, in addition it shows rapid growth and an a nice shape. ‘Arno’, therefore, could be used for both ornamental and productive aims. Inventors: Mittempergher Lorenzo, Santini Alberto, Ferrini Fabio, Fagnani Alberto N° First Deposit: RM 2006 NV 000004 Date of first deposit: 02/05/2006 N° European Deposit: 2008/0666 Date of European Deposit: 25/03/2008 Bibliographic Reference: Santini, A.; Fagnani, A.; Ferrini, F.; Ghelardini, L.; Mittempergher, L. 2007. ‘Fiorente’ and ‘Arno’ Elm trees. Hortscience. 42: 712–714.
Ulmus ‘Fiorente’, an elm cultivar resistant to Dutch elm disease (DED)
‘Fiorente’ was selected from seedlings obtained by controlled pollination of U. pumila S.10 with U. minor C.02. Resistance to DED was verified by checking symptoms after artificial inoculations in two different years on 10 3-years-old rooted cuttings. ‘Fiorente’ is monocormic and shows exceptionally rapid growth, significantly greater than the other cultivars cultivated at the same site, suggesting that it could also be used for timber or biomass production. Its habit is conical with pronounced apical dominance, a result of the limited lateral branching on the developing shoots of the current season’s growth. The crown is therefore slender and columnar. The name ‘Fiorente’ means ‘‘flourishing’’ and was chosen because of the tree’s fast and lush growth, and also because it recalls the ancient name of the city of Florence (Florentia, Fiorenza, Firenze). The main advantage in using ‘Fiorente’ is its resistance to DED, in addition it shows exceptional rapid growth and an elegant shape. ‘Fiorente’, therefore, could be used for both ornamental and productive aims. It should also be noted that strong winds have not injured any of this selection. Inventors: Mittempergher Lorenzo, Santini Alberto, Ferrini Fabio, Fagnani Alberto N° First Deposit: RM 2006 NV 000005 Date First Deposit: 02/05/2006 N° European patent: 2008/0667 Date European Deposit: 28/03/2008 Bibliographic Reference: Santini, A.; Fagnani, A.; Ferrini, F.; Ghelardini, L.; Mittempergher, L. 2007. ‘Fiorente’ and ‘Arno’ Elm trees. Hortscience. 42: 712–714.
Ulmus ‘Morfeo’, an elm cultivar resistant to Dutch elm disease (DED)
‘Morfeo’ was selected from seedlings obtained by controlled pollination of the Heybroek’s ‘405’ hybrid elm [(U. glabra x U. minor) x U. minor] with an individual of U. chenmoui. ‘Morfeo’ is a robust, fast-growing tree able to freestand at a very early age; in optimal conditions, it achieved annual increments of 164 cm in height and 2.5 cm in stem diameter. From data obtained in the comparative trial, ‘Morfeo’ growth is more strongly influenced by environment than other clones, showing a marked preference for the deep alluvial soils According to the detailed observations made in southern England (Brookes 2010), it also grew rapidly and adapted well to the maritime, winterwet climate where temperatures are moderated by the gulfstream. The crown is narrow-vase shaped with ascending branches. The stem commences forking at between 1.5 and 2 m from the ground. The levels of resistance to DED did not differ significantly from those of ‘Sapporo Autumn Gold’ but were much higher than those of ‘Commelin’ during the inoculation year. Until 2010, ‘Morfeo’ ramets were regularly propagated, planted and checked for symptoms of DED, but not one showed wilting or dieback and their susceptibility to elm leaf beetle has been shown to be lower than that of U. minor and U. pumila. It should also be noted that in England, Atlantic gales have not damaged any of this selection, and that root system has developed strongly to give exemplary resistance to wind rock (Brookes 2010). The tree readily suckers from the roots after 4–5 years. Inventors: Santini Alberto, Pecori Francesco, Ferrini Fabio N° European deposit: 2011/0223 Date of European Deposit: 02/04/2011 Santini, A.; Pecori, F.; Pepori, A.; Brookes, A. 2012. ‘Morfeo’ elm: a new variety resistant to Dutch elm disease. Forest Pathology 42: 171-176.