The project targets some poorly known aspects of FD through the application of innovative analytical techniques together with an integrated approach that covers the three partners involved in the disease: phytoplasma, vector and plant. In particular:
- The phytoplasma will be studied through the development of new genetic markers and a validation of recently of a recently published protocol for in vitro cultivation of the pathogen
- The activity of the carrier will be analyzed through epidemiological studies detailed at the molecular level, and with the study of symbionts potentially involved in control FD infectivity
- Induction of recovery will be addressed in plants grown under controlled condition
- The reactions of the vine to phytoplasmoses will be studied through analysis based on transcriptomic sequencing of mRNA (RNA-Seq) and through analysis of epigenetic modification
In the framework of INTEFLAVI project, innovative techniques such as transcriptome sequencing (RNA-Seq), metiloma sequencing, LC-MS and GC-MS analysis of the secondary metabolome will be applied, for the first time, to the study of grapevine phytoplasmoses.
The project will be conducted by three research groups (University of Turin: Plant Physiology and Agricultural Entomology, and IPSP-CNR) with extensive experience in the study of the interactions between plants and stress, relationships between vector and phytoplasma, characterization of phytoplasma and its effects on the plant. The project will produce both scientific findings on the biology of the interaction, and application results that can be immediately subjected to verification and prototyping .
The project lasts 36 months.