Umberto Bernardo

My research activity is aimed to improving the eco-sustainable control of pest insects.

• The first step is a complete characterization of the Multiorganismal insects (Holobionts).
Characterization is carried out using an integrative approach studying:
Morphology: at individual and population level (Uni and Multivariate Morphometric Analysis);
Biology: host range, modality of reproduction, occurrence of microbe-induced reproductive manipulations, parasitization behavior, reproductive potential on specific hosts and other biological traits;
Genetic: mitochondrial and nuclear genes analysis, phylogenetic reconstruction, phylogeographic analysis and methods of species delimitation, development of specific molecular markers, karyological analysis, genetic characterization of endosymbionts bacteria manipulators of reproduction (in particular PI Rickettsia).

• Use of parasitoids in classical, augmentative and conservative biological control.

Insect currently under study are both pests: a) the cynipid gall wasp Dryocosmus kuriphilus, a major pest of chestnut species; the South American tomato leafminer, Tuta absoluta; the walnut husk fly, Rhagoletis completa; the North American walnut leafminer, Coptodisca lucifluella; the blue-gum chalcid, Leptocybe invasa; the olive fruit fly, Bactrocera oleae; the Mediterranean fruit fly (Medfly), Ceratitis capitata; and b) the complex of parasitoids that control them (Eulophidae wasps, in particular).


Articles and book chapters since 2008

  • 2018 - Resistance of a Local Ecotype of Castanea sativa to Dryocosmus kuriphilus (Hymenoptera: Cynipidae) in Southern Italy - Nugnes F., Gualtieri L., Bonsignore C.P., Parillo R., Annarumma R., Griffo R., Bernardo U. - Forests , volume 9
  • 2018 - Do Torymus sinensis (Hymenoptera: Torymidae) and agroforestry system affect native parasitoids associated with the Asian chestnut gall wasp? - Ferracini C., Bertolino S., Bernardo U., Bonsignore C.P., Faccoli M., Ferrari E., Lupi D., Maini S., Mazzon L., Nugnes F., Rocco A., Santi F., Tavella L. - Biological Control , 121: 36-43
  • 2018 - Effects of environmental parameters on the chestnut gall wasp and its complex of indigenous parasitoids - Bonsignore C.P., Bernardo U. - Naturwissenschaften , volume 105
  • 2018 - The present pest status of Eucalyptus sap-suckers and gall wasps in Campania (Italy) - Garonna A.P., Bernardo U., Gualtieri L., Laudonia S., Nugnes F. - Redia , 101: 73-79
  • 2018 - A New Gall Midge Species of Asphondylia (Diptera: Cecidomyiidae) Inducing Flower Galls on Clinopodium nepeta (Lamiaceae) From Europe, its Phenology, and Associated Fungi - Bernardo U., Nugnes F., Gualtieri L., Nicoletti R., Varricchio P., Sasso R. Viggiani G. - Environmental Entomology , volume 20
  • 2017 - Life inside a gall: closeness does not favour horizontal transmission of Rickettsia between a gall wasp and its parasitoid - Gualtieri L., Nugnes F., Nappo A.G., Gebiola M., Bernardo U. - Fems Microbiology Ecology , volume 7
  • 2017 - Cinipide del castagno, cresce il controllo biologico in Campania - Bernardo U., Nugnes F., Gualtieri L., Scarpato S., Gargiulo G., Griffo R. - L'Informatore Agrario, volume 27
  • 2017 - An integrative approach to species discrimination in the Anagrus atomus group sensu stricto (Hymenoptera: Mymaridae), with a description of a new species - Nugnes F., Bernardo U., Viggiani G. - Systematics and biodiversity, 1-18
  • 2017 - A new species of Soikiella Nowicki (Hymenoptera: Trichogrammatidae) from Italy - Bernardo U., Gualtieri L., Nugnes F., Verdolini E., Riolo P., Viggiani G. - Zootaxa , 4242: 185-192
  • 2016 - No evidence of parthenogenesis-inducing bacteria involved in Thripoctenus javae thelytoky: an unusual finding in Chalcidoidea - Monti M.M., Nugnes F., Gualtieri L., Gebiola M., Bernardo U. - Entomologia Experimentalis Et Applicata , 160: 292-301
  • 2016 - When exotic biocontrol agents travel without passport: first record of Quadrastichus mendeli, parasitoid of the blue-gum chalcid Leptocybe invasa, in Italy - Nugnes F., Gebiola M., Gualtieri L., Russo E., Sasso R., Bernardo U. - Bulletin Of Insectology , 69: 85-91
  • 2015 - Genetic diversity of the invasive gall wasp Leptocybe invasa (Hymenoptera: Eulophidae) and of its Rickettsia endosymbiont, and associated sex-ratio differences - Nugnes F., Gebiola M., Monti M.M., Gualtieri L., Giorgini M., Wang J., Bernardo U. - PloS one, 10(5): e0124660
  • 2015 - Molecular phylogenetic analyses and morphological variation point to taxonomic problems among four genera of parasitoid doryctine wasps (Hymenoptera : Braconidae) - Gebiola M., Garonna A.P., Bernardo U., Belokobylskij S.A. - Invertebrate Systematics , 29: 591-609
  • 2015 - An integrative study of Necremnus Thomson (Hymenoptera: Eulophidae) associated with invasive pests in Europe and North America: Taxonomic and ecological implication - Gebiola M., Bernardo U., Ribes A., Gibson G. - Zoological journal of the Linnean Society, 173: 352-423
  • 2015 - Characterization, distribution, biology and impact on Italian walnut orchards of the invasive North-American leafminer Coptodisca lucifluella (Lepidoptera: Heliozelidae) - Bernardo U., van Nieukerken E.J., Sasso R., Gebiola M., Gualtieri L., Viggiani G. - Bulletin of Entomological Research, 105:210-224
  • 2015 - Rapid diagnosis of the invasive wax scale, Ceroplastes rusci Linnaeus (Hemiptera: Coccoidea: Coccidae) using nested PCR - Deng J., Wang X.B., Yu F., Zhou Q.S., Bernardo U., Zhang Y.Z., Wuet S.A. - Journal of Applied Entomology, 139:314-319
  • 2014 - Metabolites produced by Gnomoniopsis castanea associated with necrosis of chestnut galls - Vinale F., Ruocco M., Manganiello G., Guerrieri E., Bernardo U., Mazzei P., Piccolo A., Sannino F., Caira S., Woo S.L., Lorito M. - Chemical And Biological Technologies In Agriculture , 1: 1-3
  • 2014 - Insects and globalization: sustainable control of exotic species in Italian agro-forestry ecosystems. Landscape Management for Functional Biodiversity - Lupi D., Bernardo U., Bonsignore C.P., Colombo M., Dindo M.L., Faccoli M., Ferracini C., Gualtieri L., Marullo R., Mazzon L., Siscaro G., Suma P., Tavella L., Maini S. - Iobc-wprs Bulletin , 100: 87-90
  • 2014 - Did the parasitoid Pnigalio mediterraneus (Hymenoptera: Eulophidae) track the invasion of the horse chestnut leafminer? - Gebiola M., Lopez-Vaamonde C., Nappo A.G., Bernardo U. - Biological Invasions , 16:843-857
  • 2013 - Native Parasitoids Associated With Dryocosmus Kuriphilus In Tuscany - Panzavolta T., Bernardo U., Bracalini M., Cascone P., Croci F., Gebiola M., Iodice L., Tiberi R., Guerrieri E. - Bulletin Of Insectology , 66:195-201
  • 2013 - Description of Synergus castaneus n. sp. (Hymenoptera: Cynipidae: Synergini) Associated with an Unknown Gall on Castanea spp. (Fagaceae) in China - Bernardo U., Gebiola M., Xiao Z., Zhu C.-D., Pujade-Villar J., Viggiani G. - Annals Of The Entomological Society Of America , 106:437-446
  • 2013 - First records of the genera Histeromerus Wesmael (Hymenoptera, Braconidae, Histeromerinae) and Ecclitura Kokujev (Hymenoptera, Braconidae, Euphorinae) in Italy - Belokobylskij S., Loni A., Lucchi A., Bernardo U. - Zookeys , 310:29-40
  • 2013 - Biology and monitoring of Dryocosmus kuriphilus on Castanea sativa in Southern Italy - Bernardo U., Iodice L., Sasso R., Tutore V. A., Cascone P., Guerrieri E. - Agricultural And Forest Entomology , 15:65-76
  • 2012 - Recruitment Of Native Parasitoids By The Exotic Pest Tuta Absoluta In Southern Italy - Zappalà L., Bernardo U., Biondi A., Cocco A., Deliperi S., Delrio G., Giorgini M., Pedata P., Rapisarda C., Tropea Garzia G., Siscaro G. - Bulletin of Insectology , 65:51-61
  • 2012 - Survey on indigenous parasitoids of the invasive exotic pest Tuta absoluta in Southern Italy - Zappalà L., Bernardo U., Biondi A., Cocco A., Deliperi S., Delrio G., Giorgini M., Pedata P.A., Rapisarda C., Tropea Garzia G., Siscaro G. - Iobc-wprs Bulletin , 80:57-62
  • 2012 - A karyological study of the genus Pnigalio Schrank (Hymenoptera: Eulophidae): Assessing the taxonomic utility of chromosomes at the species level - Gebiola M., Giorgini M., Navone P., Bernardo U. - Bulletin of Entomological Research , 102:43-50
  • 2012 - First record of a walnut shield bearer Coptodisca (Lepidoptera: Heliozelidae) in Europe - Bernardo U., Sasso R., Gebiola M., Viggiani G. - Journal of Applied Entomology , 136:638-640
  • 2012 - Integration of molecular, ecological, morphological and endosymbiont data for species delimitation within the Pnigalio soemius complex (Hymenoptera: Eulophidae) - Gebiola M., Gomez-zurita J., Monti M.M., Navone P., Bernardo U. - Molecular Ecology , 21:1190-1208
  • 2012 - Gestire il cinipide del castagno mediante i nemici naturali - Guerrieri E., Bernardo U., Cascone P., Iodice L., Gebiola M., Griffo R., Pesapane G. - L'Informatore Agrario , 6:75
  • 2011 - Minatrice fogliare segnalata in Italia su noce - Bernardo U., Sasso R., Gebiola M., Viggiani G. - L'informatore Agrario , 46: 64-65
  • 2010 - A reevaluation of the generic limits of Pnigalio Schrank based on molecular and morphological evidence - Gebiola M., Bernardo U., Burks R. - Zootaxa , 2484:35-44
  • 2010 - Rickettsia symbionts cause parthenogenetic reproduction in the parasitoid wasp Pnigalio soemius (Hymenoptera: Eulophidae) - Giorgini M., Bernardo U., Monti M.M., Nappo A.G., Gebiola M. - Applied And Environmental Microbiology, 76:2589-2599
  • 2009 - Pnigalio agraules (Walker) and Pnigalio mediterraneus Ferrière and Delucchi (Hymenoptera: Eulophidae): two closely related valid species - Gebiola M., Bernardo U., Monti M.M., Navone P., Viggiani G. - Journal of Natural History , 43:2465-2480
  • 2009 - Pomodoro E Tuta Absoluta. - Bernardo U., Iodice L., Sasso R. - Coltivare Insieme , 3:12-13
  • 2008 - Karyotype of Baryscapus silvestrii Viggiani et Bernardo (Hymenoptera: Eulophidae) - Gebiola, M., Bernardo, U. - Bollettino Del Laboratorio Di Entomologia Agraria Filippo Silvestri , 62: 39-43
  • 2008 - Effects of cold storage on Thripobius javae (=T. semiluteus) (Hymenoptera: Eulophidae) - Bernardo U., Iodice L., Sasso R., Pedata P.a. - Biocontrol Science And Technology , 18: 921-933
  • 2008 - Species status of two populations of Pnigalio soemius (Hymenoptera: Eulophidae) reared from two different hosts: an integrative approach - Bernardo U., Monti M.M., Nappo A.G., Gebiola M., Russo A., Pedata P.a., Viggiani G. - Biological Control , 46: 293-303