Giorgio Gambino

Research activity:

Study of the physiological and molecular bases underlying grapevine-virus-fungal pathogen interactions. The fungal diseases downy mildew (causal agent Plasmopara viticola) and powdery mildew (Erysiphe necatrix), and some viruses (GRSPaV, GVB and GFLV), very widespread in grapevine, are used as model systems in our studies. We are investigating the modifications induced by these pathogens in the eco-physiological traits, in the gene expression (transcriptomic analyses: RNA-seq and qRT-PCR), and in the small RNA pathways (miRNAs and vsiRNA) of infected grapevines. Particular attention is paid to the complex interactions between virus-plant and water stress.

Genetic characterization of the grapevine cultivar Nebbiolo through the genome sequencing of 3 Nebbiolo clones. These data are the starting point for in-depth studies on i) interactions between the grapevine genetic biodiversity and the environment (terroir) by transcriptomic (RNA-seq) and epigenomic (Bs-seq) approaches; ii) identification of clone-specific genetic markers (Single-Nucleotide Polymorphism, SNPs) to improve the traceability of the grapevine propagation material, and the clonal identification. Particular attention is paid to new SNPs belonging to coding sequences, therefore potentially representing functional markers associated to interesting genes.

Characterization of plastic effects of somatic embryogenesis in grapevine, a regeneration system known to exploit genotype-dependent reactions to growth conditions. In-depth molecular studies will allow to identify some markers, i.e. genes, transcripts, splicing variants and epigenetic modifications, responsible for different embryogenic aptitudes in different grapevine cultivars. Somatic embryogenesis is currently used in our lab for the elimination of virus and viroid infections, for genetic transformation and genome editing approaches.

Genetic transformation of grapevine and model plants (tobacco and Arabidopsis) for functional studies.

Detection of viruses and viroids by serological and molecular techniques.


Articles and book chapters since 2008

  • 2018 - Grapevine (Vitis spp.) - Gribaudo I., Gambino G. - in "Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants", 151-160
  • 2018 - miRVIT: A novel miRNA database and its application to uncover vitis responses to flavescence dorée infection - Chitarra W., Pagliarani C., Abbà S., Boccacci P., Birello G., Rossi M., Palmano S., Marzachì C., Perrone I., Gambino G. - Frontiers in plant science, volume 9
  • 2018 - Il genoma del Nebbiolo - Gambino G., Gribaudo I. - Millevigne, 3: 6-8
  • 2018 - Dal genoma del Nebbiolo un nuovo impulso alla ricerca - Gambino G., Gribaudo I. - VQ vite, vino & qualità, 4: 42-43
  • 2018 - A viral suppressor modulates the plant immune response early in infection by regulating miRNA activity - Pertermann R, Tamilarasan S., Gursinsky T., Gambino G., Schuck J., Weinholdt C., Lilie H., Grosse I., Golbik R., Pantaleo V. Behrens SE. - Mbio , volume 9
  • 2018 - Dissecting interplays between Vitis vinifera L. and grapevine virus B (GVB) under field conditions - Chitarra W., Cuozzo D., Ferrandino A., Secchi F., Palmano S., Perrone I., Boccacci P., Pagliarani C., Gribaudo I., Mannini F., Gambino G. - Molecular Plant Pathology , 19: 2651-2666
  • 2017 - Grapevine Grafting: Scion Transcript Profiling and Defense-Related Metabolites Induced by Rootstocks - Chitarra W., Perrone I., Avanzato C.G., Minio A., Boccacci P., Santini D., Gilardi G., Siciliano I., Gullino M.L., Delledonne M., Mannini F., Gambino G. - Frontiers in plant science, 8: 654
  • 2017 - Cultivar-specific gene modulation in Vitis vinifera: Analysis of the promoters regulating the expression of WOX transcription factors - Boccacci P., Mela A., Pavez Mina C., Chitarra W., Perrone I., Gribaudo I., Gambino G. - Scientific reports, 7: 45670
  • 2017 - Cloni di vitigni piemontesi selezionati e registrati dall’IPSP-CNR nel periodo 2007-2016 - Mannini F., Gambino G., Mollo A., Santini D. - ,
  • 2017 - Ozone Improves the Aromatic Fingerprint of White Grapes - Rio Segade S., Vilanova M., Giacosa S., Perrone I., Chitarra W., Pollon M., Torchio F., Boccacci P., Gambino G., Gerbi V., Rolle L. - Scientific reports, volume 7
  • 2017 - Whole-genome sequencing and SNV genotyping of ‘Nebbiolo’ (Vitis vinifera L.) clones - Gambino G., Dal Molin A., Boccacci P., Minio A., Chitarra W., Avanzato C.G., Tononi P., Perrone I., Raimondi S., Schneider A., Pezzotti M., Mannini F., Gribaudo I., Delledonne M. - Scientific reports, volume 7
  • 2017 - Epigenetics in Plant-Pathogen Interactions - Gambino G., Pantaleo V. - Publisher: Springer International Publishing, 385-404
  • 2016 - VvPIP2;4N aquaporin involvement in controlling leaf hydraulic capacitance and resistance in grapevine - Vitali M., Cochard H., Gambino G., Ponomarenko A., Perrone I., Lovisolo C. - Physiologia Plantarum , 158: 286-296
  • 2016 - Novel functional microRNAs from virus-free and infected Vitis vinifera plants under water stress - Pantaleo V., Vitali M., Boccacci P., Miozzi L., Cuozzo D., Chitarra W., Mannini F., Lovisolo C., Gambino G. - Scientific Reports (Nature Publishing Group), 6: 20167
  • 2016 - Insights on the impact of arbuscular mycorrhizal symbiosis on tomato tolerance to water stress - Chitarra W., Pagliarani C., Maserti B.e., Lumini E., Siciliano I., Cascone P., Schubert A., Gambino G., Balestrini R., Guerrieri E. - Plant Physiology , 171: 1009-1023
  • 2016 - Grapevine-virus-environment interactions: An intriguing puzzle to solve - Perrone I., Chitarra W., Boccacci P., Gambino G. - New Phytologist ,
  • 2016 - Arbuscular mycorrhizal symbiosis-mediated tomato tolerance to drought - Chitarra W., Maserti B.E., Gambino G., Guerrieri E., Balestrini R. - Plant signalling & behavior,
  • 2015 - Interventi per il recupero e la caratterizzazione delle cultivar autoctone calabresi Castiglione e Toccarino - Mannini F., Schneider A., Gambino G., Santini D., Raimondi S., Cuozzo D., Lanati D., Marchi D., Librandi N., De Santis D. - Interventi per il recupero e la caratterizzazione delle cultivar autoctone calabresi Castiglione e Toccarino - Editor: Librandi S.p.A., Cirò M. (KR) (ITA),
  • 2015 - Multiplex rt-pcr method for the simultaneous detection of nine grapevine viruses - Gambino G. - Plant Virology Protocols: New Approaches to Detect Viruses and Host Responses, 3rd Edition - Editor: Humana Press Inc., Totowa (USA), 39-47
  • 2015 - Studio per l’individuazione dei portinnesti più adatti alla coltivazione del Gaglioppo - Mannini F., Schneider A., Gambino G., Boccacci P., Santini D., Chitarra W., Perrone I. - Librandi S.p.A., Cirò M. (KR) (ITA),
  • 2015 - Homeologs of the Nicotiana benthamiana antiviral ARGONAUTE1 show different susceptibilities to microRNA168-mediated control - Gursinsk T., Pirovano W., Gambino G., Friedrich S., Behrens S.E., Pantaleo V. - Plant Physiology (Online), 168:938-952
  • 2014 - Functional genomics in fruit trees - Gambino G., Pagliaran, C., Gribaudo I. - Tree Biotechnology, 583-613
  • 2014 - Functional genomics in fruit trees. - Gambino G., Pagliarani C., Gribaudo I. - In: K.G. Ramawat, J-M. Mérillon, M.R. Ahuja (eds.) Tree Biotechnology. Science Publishers, an imprint of CRC press (Taylor & Francis group co.), Boca Raton, FL, USA., 578-607
  • 2014 - Survey on viroids infecting grapevine in Italy: Identification and characterization of Australian grapevine viroid and Grapevine yellow speckle viroid 2 - Gambino G.,Navarro B.,Torchetti E.M.,La Notte P.,Schneider A.,Mannini F.,Di Serio F. - European Journal Of Plant Pathology , 140:199-205
  • 2014 - Construction of a synthetic infectious cDNA clone of Grapevine Algerian latent virus (GALV-Nf) and its biological activity in Nicotiana benthamiana and grapevine plants - Lovato A. ,Faoro F., Gambino G., Maffi D., Bracale M., Polverari A. ,Santi L. - Virology Journal , 11:186
  • 2013 - Hydrogen peroxide accumulation and transcriptional changes in grapevines recovered from flavescence dorée disease - Gambino G., Boccacci P., Margaria P., Palmano S., Gribaudo I. - Phytopathology, 103:776-784
  • 2013 - Genome-wide identification of viral and host transcripts targeted by viral siRNAs in Vitis vinifera - Miozzi L., Gambino G., Burgyan J., Pantaleo V. - Molecular Plant Pathology, 14:30-43
  • 2012 - The grapevine root-specific aquaporin VvPIP2;4N controls root hydraulic conductance and leaf gas exchange under well watered conditions but not under water stress - Perrone I., Gambino G., Chitarra W., Vitali M., Pagliarani C., Riccomagno N., Balestrini R., Kaldenhoff R., Uehlein N., Gribaudo I., Schubert A., Lovisolo C. - Plant Physiology , 160:965-977
  • 2012 - Co-evolution between Grapevine rupestris stem pitting associated virus and Vitis vinifera L. leads to decreased defence responses and increased transcription of genes related to photosynthesis - Gambino G., Cuozzo D., Fasoli M., Pagliarani C., Vitali M., Boccacci P., Pezzotti M., Mannini F. - Journal of Experimental Botany, 63:5919-5933
  • 2012 - Genetic transformation of fruit trees: current status and remaining challenges - Gambino G., Gribaudo I. - Transgenic Research, 21:1163-1181
  • 2012 - Co-evolution between Grapevine rupestris stem pitting-associated virus and Vitis vinifera L. leads to decreased defence responses and increased transcription of genes related to photosynthesis. - Gambino G., Cuozzo D., Fasoli M., Pagliarani C., Vitali M., Boccacci P., Pezzotti M., Mannini F. - JOURNAL OF EXPERIMENTAL BOTANY, 63:5919-5933
  • 2011 - Non-coding RNAs and gene silencing in grape - Carra A., Gambino G., Urso S., Nervo G. - Non Coding Rnas In Plants, Rna Technologies , Publisher: Springer-Verlag
  • 2011 - Somatic embryogenesis efficiently eliminates viroid infections from grapevines - Gambino G., Navarro B., Vallania R., Gribaudo I., Di Serio F. - European Journal Of Plant Pathology (10845J0), 130: 511-519
  • 2011 - Characterization of expression dynamics of WOX homeodomain transcription factors during somatic embryogenesis in Vitis viniferaEmbryogenesis In Vitis Vinifera. - Gambino G., Minuto M., Boccacci P., Perrone I., Vallania R., Gribaudo I. - Journal Of Experimental Botany (53988J0), 62: 1089-1101
  • 2011 - Functional effect of grapevine 1-deoxy-D-xylulose 5-phosphate synthase substitution K284N on Muscat flavour formation - Battilana J., Emanuelli F., Gambino G., Gribaudo I., Gasperi F., Boss P., Grando M.S. - Journal Of Experimental Botany (53988J0), 62: 5497-5508
  • 2010 - Transgene silencing in grapevines transformed with GFLV resistance genes: analysis of variable expression of transgene, siRNAs production and cytosine methylation - Gambino G., Perrone I., Carra A., Chitarra W., Boccacci P., Torello Marinoni D., Barberis M., Maghuly F., Laimer M., Gribaudo I. - Transgenic Research, 19:17-27
  • 2010 - Small RNA extraction and expression analysis by Northern blot - Batelli G., Gambino G., Mica E., Schubert A., Carra A. - In: Methodologies And Results In Grapevine Research, S. Delrot Et Al (eds), Publisher: Springer Science+Business Media B.V., Dordrecht
  • 2010 - In situ localization of Grapevine fanleaf virus and phloem-restricted viruses in embryogenic callus of Vitis vinifera - Gambino G., Vallania R., Gribaudo I. - European Journal Of Plant Pathology, 127:557-570
  • 2010 - Field Assessment And Diagnostic Methods For Detection Of Grapevine Viruses - Gambino G., Angelini E., Gribaudo I. - In: Methodologies And Results In Grapevine Research, S. Delrot Et Al (eds), Publisher: Springer Science+Business Media B.V., Dordrecht
  • 2010 - Transgene Silencing In Plants: Mechanisms, Applications And New Perspectives - Pagliarani C., Perrone I., Carra A., Gambino G. - In: Gene Silencing: Theory, Techniques And Applications, A.J. Catalano (eds), Publisher: Nova Science Publishers , NY, USA
  • 2010 - Virit: Monitoraggio Della Diffusione Di Reinfezioni Virali Dopo L’impianto Del Vigneto Con Viti Risanate. Virusvite: Diffusione Di Reinfezioni Nel Vigneto E Ruolo Delle Cocciniglie Vettrici Di Virus - Gribaudo I., Mannini F., Gambino G., Cuozzo D., Argamante N, Bosco D., Bertin S., Della Valle D., Cotroneo A. - Quaderni Della Regione Piemonte - Agricoltura , 72:209-212
  • 2010 - Viplasmi: Tecniche di riduzione del danno: termoterapia in acqua del materiale di moltiplicazione viticolo - Mannini F., Gribaudo I., Cuozzo D., Gambino G. - Quaderni Della Regione Piemonte - Agricoltura , 72:203-207
  • 2009 - Application of a site-specific DNA excision strategy for marker gene removal during gene transfer in Vitis spp. - Martinellii L., Dalla Costa L., Vaccari I., Poletti V., Gribaudo I., Gambino G., Guzzo F., Saldarelli P., Minafra A., Turturo C. - Acta Horticulturae , 827:399-403
  • 2009 - Molecular characterization of wild grape populations from north western Italy and their genetic relationship with cultivated varieties - Schneider A., Torello Marinoni D., Raimondi S., Boccacci P., Gambino G. - Acta Horticulturae , 827:211-216
  • 2009 - Cloning and characterization of small non-coding RNAs from grape - Carra A., Mica E., Gambino G., Pindo M., Maser C., Pe E., Schubert A. - Plant Journal, 59:750-763
  • 2009 - Assessment of genetic fidelity in regenerants from two Vitis vinifera cultivars - Gribaudo I., Torello Marinoni D., Gambino G., Mannini F., Akkak A., Botta R. - Acta Horticulturae , 827:131-135
  • 2009 - Characterization of T-DNA insertions in transgenic grapevines obtained by Agrobacterium -mediated transformation - Gambino G., Chitarra W., Maghuly F., Laimer M., Boccacci P., Torello Marinoni D., Gribaudo I. - Molecular Breeding, 24:305-320
  • 2009 - Monitoring the spread of viruses after Vineyard replanting with heat-treated clones of Vitis vinifera ‘nebbiolo’ - Gribaudo I., Gambino G., Bertini S., Bosco D., Cotroneo A., Mannini F. - Journal of Plant Pathology, 91:633-636
  • 2009 - Elimination of Grapevine fanleaf virus from three Vitis vinifera cultivars by somatic embryogenesis - Gambino G., Di Matteo D., Gribaudo I. - European Journal of Plant Pathology, 123:57-60
  • 2008 - A rapid and effective method for RNA extraction from different tissues of grapevine and other woody plants - Gambino, G., Perrone, I., Gribaudo, I. - Phytochemical Analysis (00205NN), 19: 520-522
  • 2008 - Viti sane e risanate unica lotta alle virosi - Gribaudo, I., Mannini, F., Cuozzo, D., Gambino, G., Argamante, N., Bertin, S., Bosco, D., Cotroneo, A. - Informatore Agrario , 46: 59-61