Cristina Marzachì

General interest

Research on the field of molecular-plant-microbe-insect interactions, with special focus of phytoplasmas. These wall-less bacteria are transmitted by phloem feeding hemipterans (cicadellids and psyllids) to several plant species where they cause economically important diseases. Control of phytoplasma diseases relies mainly on insecticide treatments to limit vector population, with deleterious impact on the environment. A better knowledge of these pathogens, their diversity in nature, the mechanism that rule their interactions with plant and insect hosts are important pre-requisites to develop new environmental friendly control strategies for phytoplasmoses.

Specific interest

Research activity in the lab focuses on the interaction among phytoplasma, plant and insect vector to identify phytoplasma genes involved in the determination of vector specificity and symptom development in the infected plant. Chrysanthemum yellows isolate of ‘Candidatus Phytoplasma asteris’ and Flavescence dorée (16SrV-C) phytoplasma are the main focus of the research. For both pathogens we have developed experimental model systems. Phytoplasma membrane genes, known hot spots for positive selection, are the target for our research. The direct and indirect effects of several abiotic and biotic elicitors of plant resistance on the interaction among plant/phytoplasma and vector are also studied, both with a field scale approach, to provide insights on the effects of these elicitors on phytoplasma epidemiology, and a laboratory scale approach, to highlight the molecular aspects of the interaction.

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Articles and book chapters since 2008

  • 2018 - Application of laser microdissection to study phytoplasma site-specific gene expression in the model plant Arabidopsis thaliana - Rossi M., Pesando M., Vallino M., Galetto L., Marzachi C., Balestrini R. - Microbiological Research , 217: 60-68
  • 2018 - miRVIT: A novel miRNA database and its application to uncover vitis responses to flavescence dorée infection - Chitarra W., Pagliarani C., Abbà S., Boccacci P., Birello G., Rossi M., Palmano S., Marzachì C., Perrone I., Gambino G. - Frontiers in plant science, volume 9
  • 2018 - Two phytoplasmas elicit different responses in the insect vector Euscelidius variegatus Kirschbaum - Galetto L., Abbà S., Rossi M., Vallino M., Pesando M., Arricau-Bouvery N., Dubrana M.-P., Chitarra W., Pegoraro M., Bosco D., Marzachì C. - Infection And Immunity , volume 86
  • 2017 - Plant teratologies as a result of phytoplasma infections - Spallino R.E., Rizza S., Oliveri C., Catara V., Marzachi C., Tessitori M. - Plant Biosystems, 151: 931-939
  • 2017 - Pest categorisation of Witches’ broom disease of lime (Citrus aurantifolia) phytoplasma - Jeger M., Bragard C., Candresse T., Chatzivassiliou E., Dehnen-Schmutz K., Gilioli G., Gregoire J.C., Miret J.J.A., MacLeod A., Navarro Navajas M., Niere B:, Parnell S., Potting R., Rafoss T., Rossi V., Urek G., Van Bruggen A., Van der Werf W., West J., Winter S., Dickinson M., Marzachì C., Hollo G., Caffier D. - EFSA Journal, 15: 5027-5049
  • 2017 - Genome sequence, prevalence and quantification of the first iflavirus identified in a phytoplasma insect vector - Abbà S., Galetto L., Vallino M., Rossi M., Turina M., Sicard A., Marzachì C. - Archives Of Virology , 162: 799–809
  • 2017 - Test performance study of isothermal amplification tests for the detection of Grapevine flavescence dorée phytoplasma and ‘Candidatus Phytoplasma solani’ - Mehle N., Kogovsek P., Constable F., De Jonghe K., Loiseau M., Veratti F., Marzachi C., Ferretti L., Angelini E., Filippin L., Sousa E., Andrade E., Hodgetts J., Dickinson M., Dermastia M. - Bulletin Oepp , 47: 18-23
  • 2017 - Effects of Grapevine leafroll-associated virus 3 on the physiology in asymptomatic plants of Vitis vinifera L. - Montero R., El Aou Ouad H., Pacifico D., Marzachì C., Castillo N., García E., Del Saz N.F., Florez-Sarasa I., Flexas J., Bota J. - Annals of Applied Biology,
  • 2017 - Pest categorisation of Palm lethal yellowing phytoplasmas - Jeger M., Bragard C., Candresse T., Chatzivassiliou E., Dehnen-Schmutz K., Gilioli G., Gregoire J.C., Miret J.A.J., MacLeod A., Navajas Navarro M., Niere B., Parnell S., Potting R., Rafoss T., Rossi V., Urek G., Van Bruggen A., Van der Werf W., West J., Winter S., Dickinson M., Marzachì C., Hollo G, Caffier D. - EFSA Journal, volume 15
  • 2017 - Identification of putative effector genes and their transcripts in three strains related to ‘Candidatus Phytoplasma aurantifolia’ - Anabestani A., Izadpanah K., Abbà S., Galetto L., Ghorbani A., Palmano S., Siampour M., Veratti F., Marzachì C. - Microbiological Research , 199: 57-66
  • 2016 - Space-time point pattern analyses of Flavescence dorée epidemic in a grapevine field: disease progression and recovery - Maggi F., Bosco D., Galetto L., Palmano S., Marzachì C. - Frontiers In Plant Science , 7: 1987-1998
  • 2016 - Transmission of Grapevine virus A and Grapevine leafroll-associated viruses 1 and 3 by Planococcus ficus and Planococcus citri fed on mixed-infected plants - Bertin S., Pacifico D., Cavalieri V., Marzachì C., Bosco D. - Annals Of Applied Biology , 53-63
  • 2016 - Transmission of Grapevine virus A and Grapevine leafroll associated virus 1 and 3 by Heliococcus bohemicus (Hemiptera: Pseudococcidae) nymphs from plants with mixed infections - Bertin S., Cavalieri V., Gribaudo I., Sacco D., Marzachì C., Bosco D. - Journal Of Economic Entomology ,
  • 2016 - Transmission of ‘Candidatus Phytoplasma asteris’ (16SrI) by Osbornellus horvathi (Matsumura 1908) co-infected with BCa. Phytoplasma phoenicium^ (16SrIX) - Rizza S., Pesce A., D'urso V., Raciti E., Marzachì C., Tessitori M. - Phytoparasitica , 44: 491-500
  • 2016 - Insect transmission of phytoplasmas - Bosco D., Marzachì C. - Vector-Mediated transmission of plant pathogens, 319-327
  • 2016 - An 1-Aminocyclopropane-1-carboxylate (ACC) deaminase-expressing endophyte increases plant resistance to flavescence dorée phytoplasma infection - Gamalero E., Marzachi C., Galetto L., Veratti F., Massa N., Bona E., Novello G., Glick B.R., Ali S., Cantamessa S., D'Agostino G., Berta G. - Plant Biosystems, 1-10
  • 2016 - Acquisition of flavescence dorée phytoplasma by Scaphoideus titanus ball from different grapevine varieties - Galetto L., Miliordos D.E., Pegoraro M., Sacco D., Veratti F., Marzachì C., Bosco D. - International journal of molecular sciences, 17: 1563
  • 2016 - Complete Genome Sequences of the Obligate Symbionts “Candidatus Sulcia muelleri” and “Ca. Nasuia deltocephalinicola” from the Pestiferous Leafhopper Macrosteles quadripunctulatus (Hemiptera: Cicadellidae). - Bennett G.M., Abbà S., Kube M., Marzachì C. - Genome Announcements, 4(1)
  • 2016 - Acibenzolar-S-methyl may prevent vector-mediated flavescence dorée phytoplasma transmission, but is ineffective in inducing recovery of infected grapevines - Miliordos D.E., Galetto L., Ferrari E., Pegoraro M., Marzachì C., Bosco D. - Pest management science,
  • 2015 - Role of the major antigenic membrane protein in phytoplasma transmission by two insect vector species - Rashidi M., Galetto L., Bosco D., Bulgarelli A., Vallino M., Veratti F., Marzachì C. - Bmc Microbiology (Online), volume 15
  • 2015 - Decreasing global transcript levels over time suggest that phytoplasma cells enter stationary phase during plant and insect colonization - Pacifico D., Galetto L., Rashidi M., Abbà S., Palmano S., Firrao G., Bosco D., Marzachì C. - Applied And Environmental Microbiology, 81:2602-2591
  • 2014 - Dynamics of acquisition and transmission of “flavescence dorée” phytoplasma in grapevine - Maggi F., Bosco D., Marzachì C. - Phytopathogenic Mollicutes , 4: 59-71
  • 2014 - Temperature-dependent transmission of Candidatus Phytoplasma asteris by the vector leafhopper Macrosteles quadripunctulatus Kirschbaum - Maggi F.,Galetto L.,Marzachi C.,Bosco D. - Memorie Della Società Entomologica Italiana , 2:87-94
  • 2014 - Acquisition capability of the grapevine Flavescence dorée by the leafhopper vector Scaphoideus titanus Ball correlates with phytoplasma titre in the source plant - Galetto L.,Miliordos D.,Roggia C.,Rashidi M.,Sacco D.,Marzachì C.,Bosco D. - Journal Of Pest Science , 87:671-679
  • 2014 - In vitro expression of phytoplasma immunodominant membrane proteins - Galetto L., Rashidi M., Yamchi A., Veratti F., Marzachì C. - Phytoplasmas and phytoplasma disease management: how to reduce their economic impact,
  • 2014 - Interactive transmission of two phytoplasmas in the vector inset - Rashidi M.,D'Amelio. R.,Galetto L.,Marzachì C.,Bosco D. - Annals Of Applied Biology , 165:404-413
  • 2014 - RNA-Seq profile of flavescence dorée phytoplasma in grapevine - Abbà S., Galetto L., Carle P., Carrère S., Delledonne M., Foissac X., Palmano S., Veratti F., Marzachì C. - BMC Genomics , 15:1088
  • 2013 - A Stage-Structured Model of Scaphoideus titanus in Vineyards - Maggi F., Marzachì C., Bosco D. - Environmental Entomology, 42:181-193
  • 2013 - Selection of reference genes from two leafhopper species challenged by phytoplasma infection, for gene expression studies by RT-qPCR - Galetto L., Bosco D., Marzachì C. - BMC Research Notes, 6:1-10
  • 2013 - Preparation of phytoplasma membrane recombinant proteins - Galetto L., Siampour M., Marzachì C. - Methods in molecular biology, 938:351-370
  • 2013 - Flavescence dorée phytoplasma titre in field-infected Barbera and Nebbiolo grapevines - Roggia C., Caciagli P., Galetto L., Pacifico D., Veratti F., Bosco D., Marzachì C. - Plant Pathology, 63:31–41
  • 2013 - Effects of two AM fungi on phytoplasma infection in the model plant Chrysanthemum carinatum - Sampò S., Massa N., Cantamessa S., D'Agostino U., Bosco D., Marzachì C., Berta G. - Agricultural and food science, 21:39-51
  • 2013 - Molecular characterization, phylogenetic comparison and serological relationship of the Imp protein of several ‘Candidatus Phytoplasma aurantifolia’ strains - Siampour M., Izadpanah K., Galetto L., Salehi M., Marzachí C. - Plant Pathology, 62:452-459
  • 2013 - Flavescence dorée phytoplasma deregulates stomatal control of photosynthesis in Vitis vinifera - Vitali M., Chitarra W., Galetto L., Bosco D., Marzachì C., Gullino M.L., Spanna F., Lovisolo, C. - Annals of Applied Biology, 162:335-346
  • 2011 - Flavescenza dorata in cv Barbera e Nebbiolo: incidenza, risanamento e suscettibilità al patogeno - Bosco D., Marzachì C. - Protezione Delle Colture , 21-23
  • 2011 - The major antigenic membrane protein of “Candidatus Phytoplasma asteris” selectively interacts with ATP synthase and actin of leafhopper vectors - Galetto L., Bosco D., Balestrini R., Genre A., Fletcher J., Marzachì C. - Plos One , 6(7): 1-12
  • 2011 - A multidisciplinary study on the effects of phloem – limited viruses on the agronomical perfomance and berry quality of Vitis vinifera cv Nebbiolo - Giribaldi M., Purrotti M., Pacifico D., Santini D., Mannini F., Caciagli P., Rolle L., Cavallarin L., Giuffrida M.G., Marzachi' C. - Journal Of Proteomics, 75: 306-315
  • 2011 - Disease note: First report of ‘Candidatus Phytoplasma asteris’ from Gerbera jamesonii in Apulia, Southern Italy - Spanò R., Marzachì C., Mascia T., Bubici G., Gallitelli D. - Journal of Plant Pathology, 93-S4.63
  • 2011 - Presenza di Tomato spotted wilt virus e ‘Candidatus Phytoplasma asteris’ su piante di gerbera in Puglia - Spanò R., Marzachì C., Mascia T., De Lucia B., Bubici G., Torchetti E.M., Rubino L., Gallitelli D. - Protezione Delle Colture , 4: 79-83
  • 2010 - Activity of benzothiadiazole on Chrysanthemum Yellows Phytoplasma (‘Candidatus Phytoplasma Asteris’) infection in daisy plants - D'Amelio R., Marzachì C., Bosco D. - Crop Protection, 29:1094-1099
  • 2010 - Effects of Pseudomonas putida S1Pf1Rif against chrysanthemum yellows phytoplasma infection - Gamalero E., D'Amelio R., Musso C., Cantamessa S., Pivato B., D'Agostino G., Duan J., Bosco D., Marzachì C., Berta G. - Phytopathology, 100:805-813
  • 2010 - Real-Time PCR diagnosis and quantification of phytoplasmas - Galetto, L., Marzachi, C. - Phytoplasmas - Genomes, Plant Hosts And Vectors , Publisher: P.G. Weintraub & P. Jones, CABI Publishing
  • 2009 - Variation in vector competency depends on chrysanthemum yellows phytoplasma distribution within Euscelidius variegatus - Galetto L., , Nardi M., Saracco P., Bressan A., Marzachì C., Bosco D. - Entomologia Experimentalis et Applicata, 131:200-207