Beatriz Navarro

Viroids and viroid diseases

Viroids are the smallest infectious agents known so far. Consisting of a small circular RNA, they may cause severe diseases to economically relevant crops (tomato, potato, peach, citrus, etc.). In contrast to viruses, viroids do not code for any protein. Therefore, the infectious process relies on the presence of structural domains in the viroid RNAs allowing them to interact directly with host factors. This feature makes viroids useful implements for getting deeper insights into the structural-functional relationships of both foreign and host RNAs.

In our laboratory, we develop multidisciplinary researches – also based on next generation sequencing (NGS) technologies – addressed to further characterize molecular bases underlying plant-viroid interaction, with major interest in unveiling the role of RNA silencing in viroid trafficking and pathogenesis and epigenetic effects of viroid infections on thoe host genome.

From an applied point of view, we focus on molecular and biological characterization viroids causing diseases in fruit trees, grapevine, vegetables and ornamental plants, on development of fast and reliable detection methods, and on the identification of control strategies of viroid diseases. In the last years we extended our activity to the identification of the new viruses and viroid-like RNAs by NGS, and to their biological and molecular characterization.


Articles and book chapters since 2008

  • 2018 - First report of avocado sunblotch viroid (ASBVd) naturally infecting avocado (Persea Americana) in Greece - Lotos L., Kavroulakis N., Navarro B., Di Serio F., Olmos A., Ruiz-Garcia A.B., Katis N.I., Maliogka V.I. - Plant disease, 102: 1470
  • 2018 - Double-Stranded RNA-Enriched preparations to identify viroids by next-generation sequencing - Navarro B., Di Serio F. - in Viral Metagenomics: Methods and Protocols, 37-43
  • 2018 - Taxonomy of the family Arenaviridae and the order Bunyavirales: update 2018 - Maes P., Alkhovsky S.V., Bao Y., Beer M., Birkhead M., Briese T., Buchmeier M.J., Calisher C.H., Charrel R.N., Choi I.R., Clegg C.S., de la Torre J.C., Delwart E., DeRisi J.L., Di Bello P.L., Di Serio F., Digiaro M., Dolja V.V., Drosten C., Druciarek T.Z., Du J., Ebihara H., Elbeaino T., Gergerich R.C., Gillis A.N., Gonzalez J.-P.J., Haenni A.-L., Hepojoki J., Hetzel U., Ho T., Hong N., Jain R.K., Jansen van Vuren P., Jin Q., Jonson M.G., Junglen S., Keller K.E., Kemp A., Kipar A., Kondov N.O., Koonin E.V., Kormelink R., Korzyukov Y., Krupovic M., Lambert A.J., Laney A.G., LeBreton M., Lukashevich I.S., Marklewitz M., Markotter W., Martelli G.P., Martin R.R., Mielke-Ehret N., Muhlbach H.-P., Navarro B., Ng T.F.F., Nunes M.R.T., Palacios G., Paweska J.T., Peters C.J., Plyusnin A., Radoshitzky S.R., Romanowski V., Salmenpera P., Salvato M.S., Sanfacon H., Sasaya T., Schmaljohn C., Schneider B.S., Shirako Y., Siddell S., Sironen T.A., Stenglein M.D., Storm N., Sudini H., Tesh R.B., Tzanetakis I.E., Uppala M., Vapalahti O., Vasilakis N., Walker P.J., Wang G., Wang L., Wang Y., Wei T., Wiley M.R., Wolf Y.I., Wolfe N.D., Wu Z., Xu W., Yang L., Yang Z., Yeh S.-D., Zhang Y.-Z., Zheng Y., Zhou X., Zhu C., Zirkel F., Kuhn J.H. - Archives of virology, 163: 2295-2310
  • 2018 - The first phlebo-like virus infecting plants: a case study on the adaptation of negative-stranded RNA viruses to new hosts - Navarro B., Minutolo M., De Stradis A., Palmisano F., Alioto D., Di Serio F. - Molecular Plant Pathology , 19: 1075-1089
  • 2018 - First Report of Grapevine Latent Viroid Infecting Grapevine (Vitis vinifera L.) in Italy - Rotunno S., Vaira A.M., Marian D., Schneider A., Raimondi S., Di Serio F., Navarro B., Miozzi L. - Plant Disease , volume 9
  • 2018 - Viroid diseases in pome and stone fruit trees and Koch’s postulates: A critical assessment - Di Serio F., Ambros S., Sano T., Flores R., Navarro B. - Viruses, 10
  • 2018 - A negative-stranded RNA virus infecting citrus trees: The second member of a new genus within the order bunyavirales - Navarro B., Zicca S., Minutolo M., Saponari M., Alioto D., Di Serio F. - Frontiers in microbiology, volume 9
  • 2017 - Viroids Infecting the Grapevine - Di Serio F., Izadpanah K., Hajizadeh M., Navarro B. - Grapevine Viruses: Molecular Biology, Diagnostics and Management, 373-392
  • 2017 - Small Circular Satellite RNAs - Navarro B., Rubino L., Di Serio F. - Viroids and Satellites, 659-669
  • 2017 - Viroid pathogenesis - Flores R., Di Serio F., Navarro B., Owens R.A. - Viroids and Satellites, 93-103
  • 2017 - Peach Latent Mosaic Viroid in Infected Peach - Flores R., Navarro B., Delgado S., Hernandez C., Xu W.-X., Barba M., Hadidi A., Di Serio F. - Viroids and Satellites, 307-316
  • 2017 - Origin and Evolution of Viroids - Di Serio F., Navarro B., Flores R. - Viroids and Satellites, 125-134
  • 2017 - Identification and characterization of privet leaf blotch-associated virus, a novel idaeovirus - Navarro B., Loconsole G., Giampetruzzi A., Aboughanem-Sabanadzovic N., Ragozzino A., Ragozzino E., Di Serio F. - Molecular Plant Pathology , 18: 925-936
  • 2017 - Engineering resistance against viroids - Flores R., Navarro B., Kovalskaya N., Hammond R.W., Di Serio F. - Current opinion in virology, 26: 1-7
  • 2017 - Actinidia chlorotic ringspot-associated virus: a novel emaravirus infecting kiwifruit plants - Zheng Y., Navarro B., Wang G., Wang Y., Yang Z., Xu W., Zhu C., Wang L., Di Serio F., Hong N. - Molecular Plant Pathology ,
  • 2017 - Chrysanthemum Chlorotic Mottle Viroid - Flores R., Gago-Zachert S., Serra P., De la Pena M., Navarro B. - Viroids and Satellites, 331-338
  • 2016 - A nuclear-replicating viroid antagonizes infectivity and accumulation of a geminivirus by upregulating methylationrelated genes and inducing hypermethylation of viral DNA - Torchetti E.M., Pegoraro M., Navarro B., Catoni M., Di Serio F., Noris E. - Scientific Reports ,
  • 2015 - Viroids, the simplest RNA replicons: How they manipulate their hosts for being propagated and how their hosts react for containing the infection - Flores R., Minoia S., Carbonell A., Gisel A., Delgado S., Lopez-carrasco A., Navarro B., Di Serio F. - Virus Research , 209: 135-145
  • 2015 - Identification and molecular characterization of a novel monopartite geminivirus associated with mulberry mosaic dwarf disease - Ma Y., Navarro B., Zhang Z., Lu M., Zhou X., Chi S., Di Serio F., Li S. - Journal Of General Virology (Print), 96:2421-2434
  • 2015 - Grapevine viroids and Grapevine fanleaf virus in North-West Iran - Hajizadeh M., Torchetti E.M., Sokhandan-Bashir N., Navarro B., Martelli G.P., Di Serio F. - Journal Of Plant Pathology (Print), 97:363-368
  • 2015 - Viroid RNA turnover: Characterization of the subgenomic RNAs of potato spindle tuber viroid accumulating in infected tissues provides insights into decay pathways operating in vivo - Minoia S., Navarro B., Delgado S., Di Serio F., Flores R. - Nucleic Acid Research, 43:2313-2325
  • 2015 - Identification and characterization of a novel geminivirus with a monopartite genome infecting apple trees - Liang P., Navarro B., Zhang Z., Wang H., Lu M., Xiao H., Wu Q., Zhou X., Di Serio F., Li S. - Journal Of General Virology (Print), 96:2411-2420
  • 2014 - Specific argonautes selectively bind small RNAs derived from potato spindle tuber viroid and attenuate viroid accumulation in vivo - Minoia S., Carbonell A., Di Serio F., Gisel A., Carrington J.C., Navarro B., Flores R. - Journal Of Virology , 88:11933-11945
  • 2014 - Viroid-like RNAs from cherry trees affected by leaf scorch disease: Further data supporting their association with mycoviral double-stranded RNAs - Minoia S.,Navarro B.,Covelli L.,Barone M.,Garcia-Becedas M.T.,Ragozzino A.,Alioto D.,Flores R.,Di Serio F. - Archives Of Virology , 159:589-593
  • 2014 - Survey on viroids infecting grapevine in Italy: Identification and characterization of Australian grapevine viroid and Grapevine yellow speckle viroid 2 - Gambino G.,Navarro B.,Torchetti E.M.,La Notte P.,Schneider A.,Mannini F.,Di Serio F. - European Journal Of Plant Pathology , 140:199-205
  • 2013 - Cytopathic effects incited by viroid RNAs and putative underlying mechanisms - Di Serio F., De Stradis A., Delgado S., Flores R., Navarro B. - Frontiers in plant science, 3:288
  • 2012 - The role of plant viroids in diseases – new developments - Di Serio F., Torchetti E.M., Flores R., Navarro, B. - CAB Reviews, 7:1-9
  • 2012 - Small RNAs containing the pathogenic determinant of a chloroplast-replicating viroid guide the degradation of a host mRNA as predicted by RNA silencing - Navarro B., Gisel A., Rodio M.E., Delgado S., Flores R., Di Serio F. - Plant Journal, 70:991-1003
  • 2012 - A single polyprobe for detecting simultaneously eight pospiviroids infecting ornamentals and vegetables - Torchetti E.M., Navarro B., Di Serio F. - Journal of Virological Methods, 186:141-146
  • 2012 - Viroids: How to infect a host and cause disease without encoding proteins - Navarro B., Gisel A., Rodio M.E., Delgado S., Flores R., Di Serio F. - Biochimie, 94:1474-1480
  • 2012 - Molecular characterization and taxonomy of grapevine leafroll-associated virus 7 - Jelkmann W., Mikona C., Turturo C., Navarro B., Rott M.E., Menzel W., Saldarelli P., Minafra A., Martelli G.P. - Archives of Virology, 157:359-362
  • 2012 - I viroidi delle colture ornamentali: emergenze fitosanitarie e misure di controllo - Di Serio F., Torchetti E.M., Navarro B. - Criticità e prospettive delle emergenze fitosanitarie , 37-49
  • 2012 - Viroids: from genotype to phenotype just relying on RNA sequence and structural motifs - Flores R., Serra P., Minoia S., Di Serio F., Navarro B. - Frontiers in Microbiology, 3:217
  • 2012 - Occurrence of Hop Stunt Viroid in Mulberry (Morus alba) in Lebanon and Italy - Elbeaino T., Abou Kubaa R., Choueiri E., Digiaro M., Navarro B. - Journal of Phytopathology, 160:48-51
  • 2012 - First report of Chrysanthemum stunt viroid in Argyranthemum frutescens in Italy - Torchetti E.M., Navarro B., Trisciuzzi V.N., Nuccitelli L., Silletti M.R., Di Serio F. - Journal of Plant Pathology, 94:451-454
  • 2011 - Somatic embryogenesis efficiently eliminates viroid infections from grapevines - Gambino G., Navarro B., Vallania R., Gribaudo I., Di Serio F. - European Journal Of Plant Pathology (10845J0), 130: 511-519
  • 2011 - Viroids and viroid diseases of plants - Flores R., Di Serio F., Navarro Ramirez B., Duran-Vila N., Owens R.A. - Studies In Viral Ecology, Volume One: Microbial And Botanical Host Systems , Publisher: Edited by Christon J. Hurst , John Wiley & Sons
  • 2011 - Citrus tristeza virus infection induces the accumulation of viral small RNAs (21 24-nt) mapping preferentially at the 3′-terminal region of the genomic RNA and affects the host small RNA profile - Ruiz-Ruiz S., Navarro B., Gisel A., PeÑa L., Navarro L., Moreno P., Di Serio F., Flores R. - Plant Molecular Biology (05842J0), 75: 607-619
  • 2011 - First record of Citrus exocortis viroid infecting Solanum jasminoides in Italy - Torchetti E.M., Navarro B., Di Serio F. - Journal of Plant Pathology, 93
  • 2010 - Viroidi: una classe di patogeni emergenti poco nota - Di Serio F., Navarro B. - Protezione Delle Colture , 4:2-8
  • 2010 - Molecular characterization of turkish isolates of Pear blister canker viroid and assestment of the sequence variability of this viroid - Yesilcollou S., Minoia S., Torchetti E.M., Kaymak S., Gumus M., Myrta A., Navarro B., Di Serio F. - Journal of Plant Pathology, 92:813-819
  • 2010 - RNA-dependent RNA Polymerase 6 Delays Accumulation And Precludes Meristem Invasion Of A Nuclear-replicating Viroid - Di Serio F., Martínez De Alba A.E., Navarro B., Gisel A., Flores R. - Journal Of Virology, 84:2477-2489
  • 2010 - Multiple viroid infections in Iranian grapevines - Hajizadeh M., Navarro B., Bashir N.S., Di Serio F. - Journal Of Plant Pathology, 92:107-122
  • 2010 - Detection And Identification Of Viroids Damaging Potato And Fruit Trees - Di Serio F., Navarro B. - Élelmiszerbiztonság Megítélési Módszerei Ii (Methods to evaluate food safety. II), Publisher: Editor: Arpad Ambrus. Publishing: Edison House Hol
  • 2009 - Deep sequencing of the small RNAs derived from two symptomatic variants of a chloroplastic viroid: implications for their genesis and for pathogenesis - Di Serio F., Gisel A., Navarro B., Delgado S., Martínez De Alba Á.E., Donvito G.. Flores R. - Plos One, 4:e7539
  • 2009 - Il viroide PSTVD minaccia pomodoro e patata - Silletti M.R., Navarro B., Bozzano G., Trisciuzzi V.n., Di Serio F - Informatore Agrario , 11:89-90
  • 2009 - Identification and characterization of Potato spindle tuber viroid infecting tomato in Italy - Navarro B., Siletti M.R., Trisciuzzi V.N., Di Serio F. - Journal of Plant Pathology, 91:723-726