Massimo Turina

In these past years I dealt with a number of different projects in various areas of phytopathology, covering viruses, fungi, and bacteria (phytoplasmas). From traditional virology I shifted my interest to the virus-host interaction both for plant viruses and for mycoviruses.

My main research interests are in the following area:

  1. Set up of diagnostic tools for detection of a vast array of plant viruses and phytoplasmas (both serological and molecular)
  2. Identification and characterization of new virus-caused plant diseases, particularly for vegetable and ornamental crops
  3. Host-pathogen interaction in the case of resistance breaking strains of Tomato spotted wilt tospovirus, in pepper and tomato
  4. Host-pathogen interaction for Ourmia melon virus, at the cellular and molecular level
  5. Virus-vector interaction for TSWV and its thrips vector Frankliniella occidentalis
  6. Mycovirus-host interaction in the case of the CHV1-Cryphonectria parasitica. In particular we are currently evaluating the effects of the virus on signal transduction pathways, in particular for the MAP kinase cascades.

Recently we have started a new project in collaboration with Mycotheca Universitatis Taurinensis (MUT) which aims at detecting and characterizing mycoviruses from a collection of marine fungi isolated from Posidonia oceanica in order to verify their possible role in some biological features of the fungus, in specific for providing a specific advantage in the adaptation to the high salinity environment of the Mediterranean waters.


Articles and book chapters since 2008

  • 2018 - The virome of the arbuscular mycorrhizal fungus Gigaspora margarita reveals the first report of DNA fragments corresponding to replicating non-retroviral RNA viruses in fungi - Turina M., Ghignone S., Astolfi N., Silvestri A., Bonfante P., Lanfranco L. - Environmental Microbiology , 20: 2012-2025
  • 2018 - First report of Peanut stunt virus on beans in Bulgaria - Pasev G., Radeva-Ivanova V., Manoussopoulos Y., Turina M., Kostova D. - New Disease Reports , 38: 9
  • 2018 - Efficient detection of Frankliniella schultzei (Thysanoptera, Thripidae) by cytochrome oxidase I gene (mtCOI) direct sequencing and real-time PCR - Urzêdo Leão E., de Almeida Spadotti D.M., Rocha K.C.G., Lima È.F.B., Tavella L., Turina M., Krause-Sakate R. - Brazilian archives of biology and technology, volume 60
  • 2018 - The Torradovirus-specific RNA2-ORF1 protein is necessary for plant systemic infection - Ferriol I., Vallino M., Ciuffo M., Nigg J.C., Zamora-Macorra E.J., Falk B.W, Turina M. - Molecular Plant Pathology ,
  • 2018 - Differenty approches to discover mycovirus associated to marine organism - Nerva L., Varese G.C., Turina M. - in: Viral Metagenomics. Methods And Protocols , pag. 97-114
  • 2018 - Identification and characterization of Hibiscus latent Fort Pierce virus in Italy - Nerva L., Vallino M., Turina M., Ciuffo M. - Journal Of Plant Pathology , 100: 145
  • 2018 - New tools to study Torradovirus molecular biology and epidemiology - Ferriol I., Turina M., Vallino M., Zamora-Macorra E.J., Nigg J.C., Falk B.W. - Acta Horticulturae , 1207: 177-184
  • 2018 - Mycoviruses mediate mycotoxin regulation in Aspergillus ochraceus - Nerva L., Chitarra W., Siciliano I., Gaiotti F., Ciuffo M., Forgia M., Varese G.C., Turina M. - Environmental Microbiology ,
  • 2018 - A Rapid Protocol of Crude RNA/DNA Extraction for RT-qPCR Detection and Quantification - Ratti C., Minguzzi S., Turina M. - Phytoplasmas, 159-169
  • 2017 - Full-length genome sequence of the tospovirus melon severe mosaic virus - Ciuffo M., Nerva L., Turina M. - Archives Of Virology , 162: 1419-1422
  • 2017 - Mycoviruses of an endophytic fungus can replicate in plant cells: Evolutionary implications - Nerva L., Varese G.C., Falk B.W., Turina M. - Scientific Reports , volume 7
  • 2017 - Genome sequence, prevalence and quantification of the first iflavirus identified in a phytoplasma insect vector - Abbà S., Galetto L., Vallino M., Rossi M., Turina M., Sicard A., Marzachì C. - Archives Of Virology , 162: 799–809
  • 2017 - ICTV virus taxonomy profile: Ourmiavirus - Turina M., Hillman B.I., Izadpanah K., Rastgou M., Rosa C., Lefkowitz E.J., Davison A.J., Siddell S.G., Simmonds P., Adams M.J., Smith D.B., Orton R.J., Sanfacon H. - Journal Of General Virology , 98: 129-130
  • 2017 - Transmission of Penicillium aurantiogriseum partiti-like virus 1 to a new fungal host (Cryphonectria parasitica) confers higher resistance to salinity and reveals adaptive genomic changes - Nerva L., Silvestri A., Ciuffo M., Palmano S., Varese G.C., Turina M. - Environmental Microbiology , 19: 4480-4492
  • 2016 - Identification of Ourmiavirus 30K movement protein amino acid residues involved in symptomatology, viral movement, subcellular localization and tubule formation - Margaria P., Anderson C.t., Turina M., Rosa C. - Molecular Plant Pathology , 17: 1063-1079
  • 2016 - Resistance to Tospoviruses in Vegetable Crops: Epidemiological and Molecular Aspects - Turina M., Kormelink R., Resende R.O. - Annual Review Of Phytopathology , 54: 347-371
  • 2016 - A TaqMan real – Time RT – PCR assay for detection of Groundnut ringspot virus - Urzedo Leao E., Tavella L., Krause-Sakate R., Turina M. - Journal Of Plant Pathology , 98: 347-350
  • 2016 - Investigation on the partial resistance of Cpkk2 knock out strain of Cryphonectria parasitica to Cryphonectria hypovirus 1 infection in presence of Geneticin and Geneticin resistance gene - Turina M., Rossi M., Moretti M. - Virus Research , 219: 58-61
  • 2016 - Host-specific accumulation and temperature effects on the generation of dimeric viral RNA species derived from the S-RNA of members of the Tospovirus genus - Bertran A., Ciuffo M., Margaria P., Rosa C., Resende R.O., Turina M. - Journal Of General Virology , 97: 3051-3062
  • 2016 - Molecular identification and biological characterization of a new potyvirus in lettuce - Ciuffo M., Mammella M., Vallino M., Caciagli P., Turina M. - Archives Of Virology , 161: 2549-2554
  • 2016 - A new tobamovirus infecting tomato crops in Jordan - Salem N., Mansour A., Ciuffo M., Falk B.W., Turina M. - Archives Of Virology , 161: 503-506
  • 2016 - Distinct Effects of p19 RNA Silencing Suppressor on Small RNA Mediated Pathways in Plants - Kontra L., Csorba T., Tavazza M., Lucioli A., Tavazza R., Moxon S., Tisza V., Medzihradszky A., Turina M., Burgyan J. - Plos Pathogens , volume 12
  • 2016 - Comparison of small RNA profiles in Nicotiana benthamiana and Solanum lycopersicum infected by polygonum ringspot tospovirus reveals host-specific responses to viral infection - Margaria P., Miozzi L., Ciuffo M., Rosa C., Axtell M.J., Pappu H.R., Turina M. - Virus Research , 211: 38-45
  • 2016 - Multiple approaches for the detection and characterization of viral and plasmid symbionts from a collection of marine fungi - Nerva L., Ciuffo M., Vallino M., Margaria P., Varese G.c., Gnavi G., Turina M. - Virus Research , 219: 22-38
  • 2016 - RNA1-independent replication and GFP expression from tomato marchitez virus isolate M cloned cDNA - Ferriol I., Turina M., Zamora-Macorra E.J. Falk B.W. - Phytopathology , 106: 500-509
  • 2015 - The first complete genome sequences of two distinct European tomato spotted wilt virus isolates - Margaria P., Miozzi L., Ciuffo M., Pappu H.r., Turina M. - Archives Of Virology , 160: 591-595
  • 2015 - Small RNA profiles of wild-type and silencing suppressor-deficient tomato spotted wilt virus infected Nicotiana benthamiana - Margaria P., Miozzi L., Rosa C., Axtell M.j., Pappu H.r., Turina M. - Virus Research , 208: 30-38
  • 2015 - The Importance of the KR-Rich Region of the Coat Protein of Ourmia melon virus for Host Specificity, Tissue Tropism, and Interference With Antiviral Defense. - Rossi M., Vallino M., Abbà S., Ciuffo M., Balestrini R., Genre A., Turina M. - Molecular Plant-Microbe Interactions, 28:30-41
  • 2015 - Evidence of a tomato spotted wilt virus resistance-breaking strain originated through natural reassortment between two evolutionary-distinct isolates - Margaria P., Ciuffo M., Rosa C., Turina M. - Virus Research , 196: 157-161
  • 2014 - Mutational analysis of two highly conserved motifs in the silencing suppressor encoded by tomato spotted wilt virus (genus Tospovirus, family Bunyaviridae - Zhai Y.,Bag S.,Mitter N.,Turina M.,Pappu H.R. - Archives Of Virology , 159:1499-1504
  • 2014 - A New Virulent Isolate of Clover Yellow Vein Virus on Phaseolus vulgaris in Bulgaria - Pasev G.,Kostova D.,Turina M. - Journal Of Phytopathology , 162:703-711
  • 2014 - The complete genome sequence of polygonum ringspot virus - Margaria P., Miozzi L., Ciuffo M., Pappu H., Turina M. - Archives Of Virology , 159:3149-3152
  • 2014 - Molecular characterization of Tomato Spotted Wult Virus in Montenegro - Zindovic J.,Ciuffo M.,Turina M. - Journal Of Plant Pathology , 96:201-205
  • 2014 - Functional characterization of the three mitogen-activated protein kinase kinases (MAP2Ks) present in the Cryphonectria parasitica genome reveals the necessity of Cpkk1 and Cpkk2, but not Cpkk3, for pathogenesis on chestnut (Castanea spp.) - Moretti M.,Rossi M.,Ciuffo M.,Turina M. - Mppol. Molecular Plant Pathology, 15:500-512
  • 2014 - Genetic dissection of a putative nucleolar localization signal in the coat protein of ourmia melon virus - Rossi M.,Genre A.,Turina M. - Archives Of Virology , 159:1187-1192
  • 2014 - The NSs protein of Tomato spotted wilt virus is required for persistent infection and transmission by Frankliniella occidentalis - Margaria P.,Bosco L.,Vallino M.,Ciuffo M.,Mautino G. C.,Tavella L.,Turina M. - Journal Of Virology , 88:5788-5802
  • 2014 - A new ilarvirus isolated from Viola x wittrockiana and its detection in pansy germoplasm by qRT-PCR - Ciuffo M., Pacifico D., Margaria P., Turina M. - Archives Of Virology , 159:561-565
  • 2013 - Protocollo diagnostico per Tomato infectious chlorosis virus e Tomato chlorosis virus su pomodoro - Manglli A.,Ciuffo M.,Mascia T.,Davino S.W.,Gallitelli D.,Turina M.,Tomassoli L. - Petria , 23:545-566
  • 2012 - Preliminary evidence of recovery from Tomato spotted wilt virus infection in Frankliniella occidentalis individuals - Mautino G.C., Sacco D., Ciuffo M., Turina M., Tavella L. - Annals of Applied Biology, 161:266-276
  • 2012 - The mycovirus CHV1 disrupts secretion of a developmentally regulated protein in Cryphonectria parasitica - Kazmierczak P., McCabe P., Turina M., Jacob-Wilk D., Van Alfen N.K. - Journal of Virology, 86:6067-6074
  • 2012 - Differential expression of the putative Kex2 processed and secreted aspartic proteinase gene family of Cryphonectria parasitica - Jacob-Wilk D., Moretti M., Turina M. Kazmierczak P., Van Alfen N.K. - Fungal Biology , 116:363-368
  • 2012 - Tospoviruses in the Mediterranean Area - Turina M., Tavella L., Ciuffo M. - Advances in Virus Research, 84:403-437
  • 2011 - Reverse Genetic Analysis of Ourmiaviruses Reveals the Nucleolar Localization of the Coat Protein in Nicotiana benthamiana and Unusual Requirements for Virion Formation - Crivelli G., Ciuffo M., Genre A., Masenga V., Turina M. - Journal Of Virology (62788J0), 85(10): 5091-5104
  • 2011 - Molecular characterization of two distinct strains of blueberry scorch virus (BlScV) in northern Italy - Moretti M., Ciuffo M., Gotta P., Prodorutti D., Bragagna P., Turina M. - Archives Of Virology (15471J0), 156(7): 1295-1297
  • 2011 - First Report of Tomato spotted wilt virus on Pepper in Montenegro - J. Zindovic, A. Bulajio, B. Kristic, M. Ciuffo, P. Margaria, M. Turina - Plant Disease (01997J0), 95(7): 882
  • 2011 - Ranunculus latent virus: a strain of artichoke latent virus or a new macluravirus infecting artichoke? - Ciuffo M., Testa M., Lenzi R., Turina M. - Archives Of Virology (15471J0), 156(6): 1053-1057
  • 2011 - Diffusione del virus BlScV sul mirtillo gigante americano - Pansa M.G., Ciuffo M., Gotta P., Frati S., Baudino M., Frontuto A., Prodorutti D., Tavella L., Turina M. - L’informatore Agrario , 34: 76-78
  • 2011 - Ourmiavirus - Rastgou M., Turina M., Milne R.G. - Virus Taxonomy Ix Reporth , Publisher: Elsevier
  • 2010 - Cloning of the glyceraldehyde 3-phosphate dehydrogenase gene of Flavescence Dorée Phytoplasma and development of serological and molecular tools for studying its expression. - Margaria P., Palmano S., Turina M. - Journal Of Phytopathology, 158:382-386
  • 2010 - Identification of Dictyothrips betae as the vector of Polygonum ring spot virus - Ciuffo M., Mautino G.C., Bosco l., Turina M., Tavella L. - Annals Of Applied Biology, 157:299-307
  • 2010 - A mutation in the Lettuce infectious yellows virus minor coat protein disrupts whitefly transmission but not in planta systemic movement - Stewart L. R., Medina V., Tian T., Turina M. - Journal Of Virology, 84:12165-12173
  • 2010 - Study of mRNA Expression by Real Time PCR of Cpkk1, Cpkk2 and Cpkk3, three MEKs of Cryphonectria parasitica, in Virus-free and Virus-infected Isogenic Isolates. - Rostagno, L., Crivelli, G., Turina, M. - Journal Of Phytopathology, 158:409-416
  • 2009 - Molecular characterization of the plant virus genus Ourmiavirus and evidence of inter-kingdom reassortment of viral genome segments as its possible route of origin - Rastgou M. , Habibi M.K., Izadpanah K., Masenga V., Milne R.G., Wolf Y.I., Koonin E.V., Turina M. - Journal of General Virology, 90:2525-2535
  • 2009 - Detection of Flavescence dorée and Bois noir phytoplasmas, Grapevine leafroll associated virus‐1 and ‐3 and Grapevine virus A from the same crude extract by reverse transcription‐RealTime Taqman assays - Margaria P., Turina M., Palmano S. - Plant Pathology, 58:838-845
  • 2009 - Agroinoculation of the Crinivirus, Lettuce infectious yellows virus, for systemic plant infection - Wang J., Turina M., Stewart L.R., Lindbo, J.A., Falk, B.W. - Virology, 392:131-136
  • 2009 - Synergistic interaction between the Potyvirus, Turnip mosaic virus and the Crinivirus, Lettuce infectious yellows virus in plants and protoplasts - Wang J., Turina M., Medina V., Falk B.W. - Virus Research, 144:163-170
  • 2009 - Segnalazione nel Parmense del virus PaMV su pomodoro - Vicchi V., Fini P., Gozzi R., Testi V., Delvago C., Turina M. - L'Informatore Agrario , 39:74-75
  • 2009 - Silencing of Kex2 significantly diminishes the virulence of Cryphonectria parasitica - Jacob-WilkD., Turina M,. Kazmierczak P., Van Alfen N.K. - Molecular Plant-Microbe Interactions, 22:211-221
  • 2009 - Characterization of Iris Yellow Spot Virus isolates from onion crops in northern Italy - Tomassoli L., Tiberini A., Masenga V., Vicchi V., Turina M. - Journal of Plant Pathology, 91: 733-739
  • 2008 - The small genomic segment of tomato spotted wilt virus isolate BR20 is necessary but not sufficient to induce lethal necrosis in Nicotiana benthamiana and local necrotic lesions in Nicotiana tabacum cv. white burley - Margaria P., Pacifico D., Ciuffo M., Turina M. - Journal Of Plant Pathology (00077NN), 90: 337-343
  • 2008 - Characterization of putative membrane protein genes of the ‘Candidatus Phytoplasma asteris’, Chrysanthemum yellows isolate - Galetto, L., Fletcher, J., Bosco, D., Turina, M., Wayadande, A., Marzachi', C. - Canadian Journal Of Microbiology (25960J0), 54(5): 341-351